How to Use a CBC Machine

Complete blood count (CBC) testing is one of the most fundamental diagnostic procedures in modern healthcare, providing critical insights into patient health through rapid, reliable blood analysis. Whether you’re working in a busy clinical laboratory, a point-of-care facility, or a veterinary diagnostic center, understanding How to Use a CBC Machine is essential for delivering accurate results efficiently. Ozelle’s innovative CBC analyzers, including the EHBT-75, EHBT-50, and EHVT-50, represent the cutting edge of hematology technology, combining advanced AI-driven cell morphology, automated processing, and intuitive user interfaces to streamline your diagnostic workflow.

This comprehensive guide will walk you through every aspect of using a CBC machine, from initial setup through result reporting, ensuring you can confidently operate these powerful diagnostic tools.

How to Use a CBC Machine：Understanding Components and Specifications Firstly

Before you begin any testing procedure, it’s important to become familiar with the key components of your CBC analyzer. Ozelle’s machines feature a sophisticated array of systems working in harmony to deliver lab-grade results.

Display and User Interface form the command center of your device. With 7–10.1 inch color touch screens offering resolutions of 800×1280 or 1024×600 pixels, these intuitive displays support multi-language navigation and provide real-time operation monitoring. The user-friendly interface eliminates complexity, allowing operators of all experience levels to perform testing with confidence.

Sample Loading Mechanism in Ozelle devices accepts both capillary and venous blood samples, utilizing user-friendly holders or disposable sample collectors. This flexibility enables safe, minimal-touch sample preparation—a critical feature for reducing cross-contamination and improving operator safety.

The Staining and Mixing Systems work automatically within the machine, delivering consistent mixing and single-use liquid-based wet staining for exceptional cell visualization. This automation removes a major source of operator error and ensures reproducible results every time.

Analysis Chambers and Optical Systems employ high-resolution digital cell morphology imaging coupled with SwissOptic® customized lenses and photoelectric colorimetry. This advanced optical architecture captures the finest cellular details necessary for accurate differential counting and anomaly detection.

Finally, Thermal Printer and Connectivity options provide flexible reporting solutions. With USB ports, LAN, WiFi, and Bluetooth integration, your CBC machine seamlessly connects to laboratory information systems (LIS), hospital information systems (HIS), and electronic health records (EHR) for real-time data management.

Preparing Your Sample: Collection and Preparation

Successful CBC testing begins with proper sample collection and handling. Ozelle’s analyzers require minimal sample volumes—just 30–60 μL for loading, with only 30 μL needed for actual analysis. This minimally invasive approach is particularly beneficial for pediatric, geriatric, and point-of-care applications.

Capillary Collection offers a simple alternative to venous draws, especially suitable for patients with difficult veins or those requiring fingertip testing. Using the provided disposable collector, a small blood sample is obtained and loaded directly into the test kit. This method reduces patient discomfort while maintaining accuracy.

Venous Collection follows standard laboratory procedures. Ensure correct fill volume per manufacturer guidelines to enable seamless automated system intake. Proper mixing and the avoidance of hemolysis or clots are critical for reliable results.

Whichever collection method you choose, prompt analysis is essential. Store samples according to manufacturer guidance, and always verify that your sample meets quality requirements before proceeding with testing.

Getting Started: Setup and Initialization

Proper machine setup ensures reliable performance from day one.

Power and Connectivity setup is straightforward. Plug in the AC cable and power up your CBC machine via the touch screen or physical button. Next, configure communication by selecting USB, LAN, WiFi, or Bluetooth connectivity options. Once connected, configure LIS integration within the menu for seamless digital data transfer. This connection allows test orders and results to flow automatically between your laboratory system and the analyzer.

Pre-Operation Checks are essential daily procedures. Begin with visual inspection and system self-test checks for all modules. Confirm that software loads properly and the display is operational. Most Ozelle machines include automated self-check diagnostics that run at startup or can be initiated manually, providing confidence that all systems are ready for testing.

Quality Control and Calibration establish the foundation for accurate results. Insert a dry-type QC card daily or per shift, following the on-screen prompts to run QC verification. Ozelle’s system automatically calibrates using AI-driven image standards at startup or upon user demand. Always review QC reports and ensure all metrics fall within the manufacturer’s acceptance range before proceeding with patient testing.

Preparing Test Kits: The Building Blocks of Accuracy

Each test in an Ozelle CBC analyzer begins with a single test kit—a carefully engineered, ready-to-use package designed for precision and safety.

Test Kit Components include a pre-calibrated counting chamber, sterile disposable blood collector, and all necessary reagents. This integrated design minimizes handling and reduces the risk of errors. Each kit is individually packaged to prevent cross-contamination and ensure sterility.

Storage and Handling of test kits is refreshingly simple. Unlike many competitors, Ozelle kits require no cold chain—room-temperature storage is perfectly adequate. Before use, always verify that kits are undamaged and within their expiration date. One kit per patient per test is the golden rule, ensuring maximum safety and accuracy.

Loading Test Kits into your machine is intuitive. Simply follow the on-screen or kit diagram instructions. The system features auto-detection with error flagging for mismatched or incorrect kits, preventing errors before they impact results. If the machine prompts an error, simply remove and reload the kit, consulting visual or software instructions as needed.

The Sample Loading Process: From Collection to Analysis

Now that your machine is ready and your test kit is in place, it’s time to load your patient sample.

Manual Sample Preparation begins with careful blood collection. Use the disposable tip to draw your sample, then gently mix to ensure uniformity. This preliminary mixing is crucial for consistent automated uptake by the machine.

Loading Into Machine starts with opening the compartment access via software or manual latch. The system will guide you through volume measurement, physically restricting over- or under-filling. Insert your collector or sample tube into the designated slot, and confirm that the screen recognizes your sample.

Automation Activation happens with a single touchscreen command. Once initiated, the machine takes complete control. The automated staining system applies Ozelle’s proprietary liquid-based cytology, delivering optimal cell staining. Simultaneous automated mixing ensures test integrity, and you can monitor the entire process in real time on your screen.

Analysis in Action: From Imaging to Results

Modern CBC analyzers like Ozelle’s machines perform analysis through sophisticated imaging and artificial intelligence.

Cell Morphology Analysis begins with imaging acquisition. Ozelle’s custom high-resolution optics capture thousands of images for AI analysis. Multispectral imaging using different light bands produces detailed cellular profiles, enabling precise detection of normal and abnormal cells.

Liquid-Based Staining Technology represents a major advance over older methods. Each test uses single-use, optimized staining with no manual steps or wash procedures. This approach prevents carryover between tests and ensures consistently clear results within minutes.

Advanced Analysis Parameters go far beyond basic CBC counts. The analyzer performs detailed WBC differential counting across 16–38 categories, including rare and abnormal cells. Simultaneously, it measures RBC counts, platelet counts, hemoglobin, and calculates important indices. The complete result set includes over 20–38 hematology parameters depending on your machine model.

The advanced hematology parameters far exceed basic complete blood count (CBC) testing. Modern analyzers like Ozelle’s product lineup deliver comprehensive automated differential analysis across numerous cell categories, combined with precise quantitative measurements of blood cell populations and their derived indices. Here is a detailed functional comparison of different Ozelle model specifications:

Ozelle Hematology Parameter Comparison

Parameter	EHBT-50 (Multi-Func)	EHBT-75 (7-Diff)	EHBT-25 (3-Diff)	EHVT-50 (Veterinary)
Total Parameters	37 Parameters	37 Parameters	21 Parameters	42 Parameters
WBC Differential	11 base + advanced ratios	11 base + advanced ratios	3-Diff	19 items
WBC Components (Basic)	WBC, NEU#, LYM#, MON#, EOS#, BAS#	WBC, NEU#, LYM#, MON#, EOS#, BAS#	WBC, GRAN, LYMPH, MID	WBC, NEU#, LYM#, MON#, EOS#, BAS#
Advanced WBC Detection	NST#, NSG#, NSH# + NLR, PLR	NST#, NSG#, NSH# + NLR, PLR	None	NST#, NSG#, NSH# + ratios
RBC Parameters	10 items: RBC, HGB, HCT, MCV, MCH, MCHC, RDW-SD, RDW-CV, RET#, RET%	10 items: RBC, HGB, HCT, MCV, MCH, MCHC, RDW-SD, RDW-CV, RET#, RET%	8 items: RBC, HGB, HCT, MCV, MCH, MCHC, RDW-SD, RDW-CV	16 items: RBC, HGB, RET#, RET%, HCT, MCV, MCH, MCHC, RDW-CV, RDW-SD, HDW-CV, HDW-SD, SPH#, SPH%, ETG#, ETG%
Platelet Parameters	6 items: PLT, MPV, PDW, PCT, P-LCR, P-LCC	6 items: PLT, MPV, PDW, PCT, P-LCR, P-LCC	6 items: PLT, MPV, PDW, PCT, P-LCR, P-LCC	7 items: PLT, MPV, PDW, PCT, APLT#, P-LCC, P-LCR
Advanced Cell Detection	ALY#, PAg#, RET, NST, NSG, NSH	ALY#, PAg#, RET, NST, NSG, NSH	None	ALY#, PAg#, RET
Additional Functions	Immunoassay + Biochemistry + Urine/Fecal	Hematology only	Hematology only	Urine + Feces + Immunoassay
Sample Volume	30µL	30µL	40µL	55µL
Throughput	10 samples/hour	10 samples/hour	12 samples/hour	8 samples/hour

AI-Powered Intelligence is the true differentiator. Ozelle’s deep learning algorithms are trained on millions of global samples, ensuring robust accuracy and consistency. The system recognizes and classifies cells with remarkable precision, highlighting any abnormal morphology that might indicate disease. Continuous algorithm updates ensure alignment with the latest clinical standards.

Reviewing and Reporting Results

Analysis Completion Time is impressively fast. Routine samples produce results within 6 minutes, with comprehensive morphological review for any AI-flagged abnormalities. With throughput of 8–12 tests per hour, your machine can handle typical laboratory volumes while maintaining quality. Both batch and STAT/priority workflows are available for different testing scenarios.

Results Visualization provides immediate insight. Your touchscreen displays the full parameter printout, morphological images, and interpretive aids all at once. Graphical and numeric output formats accommodate different preferences, and trending options allow easy comparison of repeat patient tests.

Report Output is flexible and professional. Built-in or external thermal and A4 printers generate lab-grade reports ready for clinician review. Customizable output options let you tailor data presentation for different clinical sites—whether you’re reporting 20 or 38 parameters.

Data Management capabilities ensure that results are never lost. Your machine retains up to 100,000 patient results with robust patient ID linkage for immediate recall. Historical data access enables trend analysis, and flexible export options via USB, network, WiFi, or Bluetooth ensure compatibility with your existing laboratory systems.

Multi-Functional Analysis and Advanced Features

Beyond basic CBC testing, Ozelle’s machines offer expanded capabilities that maximize your diagnostic potential.

Hematology-Only Mode is enabled by default, delivering streamlined CBC workflows for rapid testing with full access to advanced reporting. AI-facilitated interpretation includes built-in reference ranges and flagging logic to guide your clinical decision-making.

Additional Testing Modules extend functionality. Many models support immunoassay testing for inflammation, cardiac markers, and hormones. Dedicated channels for urine and fecal testing minimize cross-test contamination. Biochemistry modules enable routine metabolic, kidney, liver, and diabetes testing on most machines.

System Integration and Troubleshooting

LIS/HIS Connectivity is simple and robust. Plug-and-play interfaces work with standard laboratory digital environments, enabling real-time reporting and two-way data exchange with your hospital systems.

Common Issues are rare but quickly resolved. Sample volume errors trigger on-screen notifications, kit recognition failures prompt clear resolution steps, and QC or communication errors provide immediate alerts with recovery advice. Most problems are corrected by reloading samples or kits or running QC again. When advanced assistance is needed, Ozelle’s integrated help system guides you to technical support.

System Recovery maintains data integrity. Safe reboots occur with no data loss, and accessible reset protocols ensure your machine quickly returns to normal operation.

Safety and Compliance

Always follow Biological Safety protocols. Wear appropriate protective equipment, handle all materials as biohazard waste, and dispose of single-use components correctly. Your CBC analyzer works with bloodborne pathogens, so vigilance is essential.

All Ozelle CBC analyzers are CE-marked and FDA-cleared, meeting strict international standards for laboratory devices. Your facility can confidently rely on these instruments for clinical decision-making.

Conclusion

How to Use a CBC Machine？an Ozelle CBC machine represents the perfect balance between advanced technology and user-friendly design. From setup through reporting, every step has been engineered for accuracy, efficiency, and safety. By mastering the procedures outlined in this guide, you’ll unlock the full potential of these powerful diagnostic tools, delivering the accurate, rapid results your patients deserve.

For additional support, training, or technical assistance, contact Ozelle Diagnostics at info@ozellepoct.com or visit www.ozellemed.com. Our dedicated global support team stands ready to ensure your success.